G(RCF)-RPM Conversion

Details

**Radius =** horizontal distance (in centimeters) from center
of rotor hub to target fluid level.

**RPM =** centrifuge rotational speed in rotations per minute.

**G-force =** relative centrifugal force (RCF) in “g”.

**RCF =** 1.118*r*(RPM/1000)^{2}

The force generated by a centrifuge depends on the rotational speed and the distance from the rotor hub to the target fluid level. Enter any two of these into the fields above and click the Calculate button next to the remaining empty field and the value for the empty field will be calculated.

The target fluid level is the level in the centrifuge tube at which your object of interest will concentrate. In the case of PRP the object is platelets. Since platelets are intermediate in density between red blood cells and plasma they will concentrate in a layer between plasma and RBC. This level is dependent on the hematocrit, but Rmid is a good approximation. See the RADIUS page for more detail.